|
KMID : 1377020140110010010
|
|
Tissue Engineering and Regenerative Medicine
2014 Volume.11 No. 1 p.10 ~ p.15
|
|
|
Immobilization of fibrinogen antibody on self-assembled gold monolayers for immunosensor applications
|
|
Cho Hong-Sik
Justin Zook
Todd Banner
Park Sang-Hyug
Min Byoung-Hyun
Karen A Hasty
Eugene Pinkhassik
Erno Lindner
|
|
|
Abstract
|
|
|
|
Self-assembled gold monolayers offer several advantages for the realization of novel modified electrodes for biosensor applications. This is due to their ability to decrease non-specific adsorption and provide for covalent attachment of biomolecules. Surfaces for these applications require the precise control of ligand density, the ability to immobilize ligands, and in situ-modulation of ligand activity. In this study, we focused our studies on the immobilization of antibody on a gold monolayer surface. We self-assembled thioctic acid onto the gold surface as an anchor point for the immobilization of anti-fibrinogen onto the surface. The modifications to the gold surface were characterized by ELISA, ellipsometry, and AFM.
|
|
|
KEYWORD
|
|
|
self-assembled monolayers (SAMs), anti-fibrinogen, immunosensors, enzyme-linked immuno sorbent assay (ELISA), ellipsometry, atomic force microscopy (AFM), gold monolayer, antibody immobilization
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
   
|